Plasmid_Backbone

Part:BBa_K5073005:Design

Designed by: Shuwen Chen   Group: iGEM24_HBMU-Taihe   (2024-10-14)


PiggyBac


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1560
    Illegal SpeI site found at 952
    Illegal PstI site found at 917
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 952
    Illegal PstI site found at 917
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BglII site found at 460
    Illegal XhoI site found at 1554
    Illegal XhoI site found at 1822
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1560
    Illegal SpeI site found at 952
    Illegal PstI site found at 917
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1560
    Illegal SpeI site found at 952
    Illegal PstI site found at 917
    Illegal NgoMIV site found at 2608
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 3784
    Illegal SapI.rc site found at 4866


Design Notes

1


Source

1

References